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Updated: Jan 13, 2026

Ultrastructural Expansion Microscopy in Three In Vitro Life Cycle Stages of Trypanosoma cruzi

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サイクリックマルチプレックス展開顕微鏡法による細胞内微細構造の解明

Seweryn Gałecki^1,2,3, Bo-Jui Chang^1,2, Felix Zhou^1,2

¹Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX 75390, USA.

bioRxiv : the preprint server for biology

|January 9, 2026

Abstract:

Despite advances in fluorescence microscopy, spectral overlap and limited resolution hinder the dense mapping of the cellular ultrastructure. To overcome these challenges, we developed Cy-ExM, a high-plex imaging strategy that integrates optimized cryo-fixation for antigen preservation, expansion microscopy, and iterative immunofluorescence labeling. Using oblique plane microscopy, we perform three-dimensional super-resolution imaging of 20 biological targets encompassing the full cellular volume of individual mammalian cells.

キーワード:

展開顕微鏡法超解像イメージング細胞生物学高プレックスイメージング 3Dイメージング

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