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Updated: Jan 13, 2026

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC

Published on: March 18, 2011

ArabidopsisにおけるLandsサイクルを介したリゾホスファチジルコリン恒常性の維持による根成長の制御

Liping Wang¹, Michael Kazachkov², Li Qin³

¹Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.

Plant & cell physiology

|January 9, 2026

Abstract:

Lysophosphatidylcholine acyltransferase (LPCAT) is a key enzyme of the Lands cycle that remodels phosphatidylcholine (PC). Proper balance between lysophosphatidylcholine (LPC) and PC is essential for phospholipid turnover and membrane homeostasis. Here, we show that the Arabidopsis lpcat double mutant exhibits an altered LPC/PC ratio in root tissues, reflecting a compromised Lands cycle. Using a chemical phenocopying strategy, we found that root growth of the lpcat mutant was more sensitive than that of wild type to lysoPAF, a structural analog of LPC. This defect was largely rescued when lysoPAF treatment was combined with ONO-RS-082, a phospholipase A2 (PLA2) inhibitor. Subcellular localization experiments with GFP fusions demonstrated that both LPCAT1 and LPCAT2 are targeted to the endoplasmic reticulum (ER) membrane. Furthermore, RNA-seq analysis of root tissues revealed transcriptional changes indicative of ER stress and altered endomembrane trafficking. Together, our results demonstrate that ER-localized LPCAT plays a pivotal role in maintaining root growth integrity when plants encounter conditions that require adjustments in lipid homeostasis. The likely role of ER localized LPCAT in modulating ER stress and root growth will be discussed.

キーワード:

Arabidopsis 小胞体膜 Landsサイクルリゾホスファチジルコリンアシル基転移酵素脂質恒常性根成長

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Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

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