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Raju S Thombal1, Vrushali H Jadhav1

  • 1Division of Organic Chemistry, National Chemical Laboratory (CSIR-NCL), Pune-411008, India. vh.jadhav@ncl.res.in.

Organic & biomolecular chemistry
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PubMed

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Production of Pharmaceuticals01:30

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...