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Overview of Fatty Acid Metabolism01:28

Overview of Fatty Acid Metabolism

Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis directly...
Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...

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関連する実験動画

Updated: Jul 7, 2026

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

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脂肪前駆細胞からの効率的なベージュ脂肪形成に向けて：脂肪由来幹細胞を用いたプロトコル最適化

Klaudia Simka-Lampa1, Agnieszka Kosowska1, Wojciech Garczorz1

  • 1Department of Biochemistry, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland.

Cells
|January 9, 2026
PubMed
まとめ

研究者らは、実験室の培養皿でベージュ脂肪細胞（脂肪細胞）を増殖させる方法を最適化した。この新しいプロトコルはヒト細胞を使用し、肥満や糖尿病治療の研究のための信頼できるモデルを提供する。

キーワード:
脂肪形成脂肪由来幹細胞ベージュ脂肪細胞褐色脂肪組織分化熱発生脱共役タンパク質1（UCP1）

さらに関連する動画

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

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関連する実験動画

Last Updated: Jul 7, 2026

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Published on: March 28, 2013

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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

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Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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科学分野:

  • 代謝研究
  • 細胞生物学
  • バイオテクノロジー

背景:

  • 褐色脂肪組織（BAT）は、肥満や2型糖尿病などの代謝性疾患の治療における主要な標的です。
  • BATの活性化と脂肪形成を研究するには、信頼できるin vitroモデルの開発が不可欠です。

主な方法:

  • ADSC分化のために30以上の脂肪形成条件をスクリーニングしました。
  • 顕微鏡検査、Oil Red O染色、およびUCP1発現（RT-qPCR、Western blot）によって分化を評価しました。
  • 様々な脂肪形成因子、誘導時間、血清濃度をテストしました。

結論:

  • ヒトADSCからのベージュ脂肪細胞への分化のための堅牢なin vitroプロトコルを開発し、検証しました。
  • ドナー間のばらつきを強調し、代謝研究における個別化アプローチの必要性を示唆しました。
  • ベージュ脂肪の生物学および潜在的な治療戦略の研究に役立つモデルを提供しました。