低塩分条件下におけるHalomonas smyrnensis IIIM VA-6を用いたエクトインの効率的生産

This study optimizes sustainable ectoine production in reduced salinity and minimal media to meet demands in pharmaceuticals, food, and cosmetics, isolating a halophilic bacterium 99.1% similarity to Halomonas smyrnensis from Sambhar Salt Lake. Optimization of ectoine production was performed using the one-variable-at-a-time method and Response Surface Methodology, assessing variables such as carbon and nitrogen sources, incubation time, pH, temperature, inoculum density, agitation rate, and salinity. High-Performance Liquid Chromatography confirmed total ectoine production, which increased from 0.42 g/L to 6.5 g/L, a 16.25-fold enhancement compared to initial unoptimized conditions. The optimal ectoine yield by Halomonas smyrnensis IIIM VA-6 was achieved after 72 hours under conditions of 5% (w/v) salinity, 10.0 g/L monosodium glutamate, pH 5.0, 10.0 g/L lactose, and 250 r.p.m. agitation, and characterized by FTIR, NMR, LC-MS. This study represents a significant improvement in sustainable production of ectoine under reduced salinity conditions using a halophilic strain.