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Updated: Jan 13, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
12:13

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Published on: October 8, 2012

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形質転換不可能な腸炎ビブリオの迅速な遺伝子工学プラットフォームへの変換

Yuxuan Zhu1, Siqi Luo1, Xu Cui1

  • 1State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Engineering Research Center for Microbial Cell Factories, Hubei Key Laboratory of Instrustrial Microbiology, School of Life Sciences, Hubei University, Wuhan 430062, P.R. China.

ACS synthetic biology
|January 9, 2026
PubMed
まとめ

研究者らは、防御システムを無効化することにより、腸炎ビブリオにおける遺伝子操作の障壁を克服しました。この成果は、効率的な分子クローニングを可能にし、急速に成長する海洋細菌を用いた新しいバイオテクノロジー応用の道を開きます。

キーワード:
DdmDEモジュール腸炎ビブリオバイオテクノロジー宿主急速増殖分子クローニングプラットフォームスタンドアロンDNase

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Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi09:01

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Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
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