Pimpinella brachycarpa抽出物のラット歯周炎モデルにおける抗炎症作用および骨保護作用

Pimpinella brachycarpa has been used in food and traditional herbal medicine for its anti-inflammatory and antimicrobial properties. This study evaluated the therapeutic potential of P. brachycarpa extract (PBE) in a rat model of ligature-induced periodontitis and identified its active compounds, such as green tea catechin (GTC), chlorogenic acid (CGA), and their combination (MIX). Seven-week-old rats received PBE (150-300 mg/kg/day) for 14 days post-ligation to induce periodontitis. Outcomes were assessed using gross morphology, histology, TRAP staining, and immunohistochemistry. UPLC revealed CGA as a major constituent in PBE. PBE significantly reduced alveolar bone loss and inflammatory responses compared to the vehicle-treated ligature-induced periodontitis group (LIG), with effects comparable to or exceeding those of ISD (Zea mays L. extract and Magnolia cortex extract-containing herbal formulaas a reference drug), GTC, CGA, and MIX. TRAP and IHC analyses indicated suppression of osteoclast activity and decreased expression of CD45, IL-1β, and TNF-α. These findings suggest that PBE mitigates periodontitis via modulation of osteoclastogenesis and immune responses. Given its efficacy and bioactive profile, PBE may serve as a promising candidate for the development of natural therapeutics against periodontal disease.