Geranium wilfordii Maxim.抽出物中のコリラギンおよびゲラニインの同時定量のための検証済みアイソクラティックHPLC-UV法

Geranium wilfordii Maxim. is a traditional medicinal plant rich in ellagitannins such as corilagin (CG) and geraniin (GR), which possess antioxidant and anti-inflammatory properties. However, accurate quantification of CG and GR in complex herbal matrices is hindered by co-eluting impurities and poor UV resolution. Here, we developed and validated a simple isocratic HPLC-UV method for their simultaneous determination in G. wilfordii extract. Separation was achieved on a Polaris 3 C18-A column (250 mm × 4.6 mm, 3 µm) using acetonitrile/0.2% formic acid in water (11:89, v/v) with UV detection at 270 nm. The method showed excellent linearity (25-300 µg/mL, R² > 0.995), precision (RSD < 2.7%), accuracy (recovery 99.5-101.2%), and low detection limits (<3 µg/mL). Previous approaches have relied on gradient HPLC or MS-based techniques, often requiring long run times, costly instrumentation, or additional purification (e.g., HSCCC). In contrast, this study demonstrates a validated isocratic method that enables baseline separation and simultaneous quantification of CG and GR in a single run. This robust and simplified analytical strategy provides a practical tool for routine quality control and phytochemical standardization, with potential applications across pharmaceutical, food, and cosmetic industries.