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関連する概念動画

Cell Signaling in Plants01:25

Cell Signaling in Plants

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Plant Tissues01:18

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Plants are multicellular eukaryotes with tissue systems made of various cell types that carry out specific functions. Different tissues work together to perform a unique function and form an organ. Organs working together form organ systems. Vascular plants have two distinct organ systems: a shoot system and a root system. The shoot system consists of two portions: the vegetative (non-reproductive) parts of the plant, such as the leaves and the stems, and the reproductive parts of the plant,...
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Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
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Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
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Updated: Jan 13, 2026

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
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植物におけるターボIDベースの近接ラベリング

Chaonan Shi1, Huang Tan2, Rosa Lozano-Durán3

  • 1Department of Plant Biochemistry, Centre for Plant Molecular Biology (ZMBP), Eberhard Karls University, Tübingen, Germany. chaonan.shi@uni-tuebingen.de.

Methods in molecular biology (Clifton, N.J.)
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PubMed
まとめ

この章では、植物タンパク質間相互作用を同定するためのターボID近接ラベリングプロトコルについて詳述する。研究者は、融合タンパク質の作成からTi-TANプラスミドコレクションを使用した結果の分析までをガイドする。

キーワード:
ニコチアナ・ベンサミアナタンパク質複合体タンパク質間相互作用近接プロテオーム近接ラベリングプロキシオームターボID

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背景:

  • 近接ラベリング（PL）技術は、生きた生物におけるタンパク質間相互作用の研究に不可欠である。
  • タンパク質複合体の理解は、植物における細胞プロセスの解明に不可欠である。

研究 の 目的:

  • 植物におけるターボIDベースの近接ラベリングのための詳細なステップバイステッププロトコルを提示する。
  • 植物の文脈におけるタンパク質の「プロキシオーム」（相互作用パートナー）の包括的な定義を可能にする。

主な方法:

  • 近接依存性ビオチン化のためのターボID酵素の利用。
  • 融合タンパク質の生成のための公開されているTi-TANプラスミドコレクションの使用。
  • 融合タンパク質の生成からデータ分析までの包括的なワークフロー。

主要な成果:

  • このプロトコルは、関心のあるタンパク質に近接したタンパク質の同定を容易にする。
  • 植物タンパク質相互作用網のマッピングのためのターボIDベースのPLの成功した応用。
  • Ti-TANプラスミドコレクションは実験設定を簡素化する。

結論:

  • このターボIDベースの近接ラベリングプロトコルは、植物の相互作用網研究のための堅牢な方法を提供する。
  • 提示されたワークフローは、研究者が生体内でタンパク質複合体を調査することを可能にする。
  • このアプローチは、植物の分子メカニズムの理解を深める。