ハキリアリ共生菌であるコウボウチャワンタケ属の菌類からのプロトプラスト作出プロトコル

¹Section for Ecology and Evolution, Department of Biology, University of Copenhagen, København, Denmark.

|January 10, 2026

Abstract:

We present a protocol to isolate viable protoplasted cells of the basidiomycete fungal mutualist (Leucoagaricus gongylophorus) obligately farmed by leafcutter ants. We describe steps for culturing actively growing mycelia, enzymatic digestion of fungal cell walls under osmotic stabilization, and purification of protoplasts (plasma membrane enclosing cytoplasm and organelles). We then detail procedures for assessing viability and wall removal using microscopy and fluorescent staining.

キーワード:

細胞生物学細胞分離環境科学進化生物学顕微鏡検査

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