アグロバクテリウム・ツメファシエンスを介した形質転換によるトランスジェニックトマト系統の作出と選抜のためのプロトコル
Fanourios Mountourakis1, Sotirios Fragkostefanakis2, Panagiotis Nikolaou Moschou3
1Department of Biology, University of Crete, 70013 Heraklion, Greece; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 70013 Heraklion, Greece.
STAR protocols
|January 11, 2026
まとめ
本研究では、アグロバクテリウム・ツメファシエンスを介した形質転換を用いた安定したトランスジェニックトマト系統の作出プロトコルを詳述する。本手法は、種子滅菌からPCRによるT-DNA挿入確認までを網羅し、信頼性の高い遺伝子改変を可能にする。
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