免疫グロブリンおよび補体タンパク質の定量のための免疫混濁測定法における酸化的干渉

Immunoturbidimetric assays are widely used to quantify immunoglobulins and complement proteins but are susceptible to interference. This study evaluated the effects of oxidative agents, including the Fenton reaction and hypochlorous acid (HOCl), on these assays. Serum pools were oxidized in vitro, and IgA, IgG, IgM, C3, and C4 were measured. Oxidative conditions increased measured concentrations of IgA, IgM, C3, and C4, whereas IgG was unaffected by HOCl. Increased oxidative stress index values and reduced thiol levels confirmed oxidative modification, indicating potential impairment of assay accuracy under oxidative stress.