タバコ抽出物環境下で刺激されたマクロファージからの炎症性サイトカインの放出
Karabi Das1, Mohit Singh Raghuvanshi2, Apurv Mehrotra3
1Department of Oral Medicine and Radiology, Regional Dental College, Guwahati, Assam, India.
Background:
Denture base materials, when exposed to carcinogenic substances like smokeless tobacco extracts (STE), may provoke inflammatory responses in oral tissues. Understanding macrophage-mediated cytokine release in such environments is critical to evaluating the biocompatibility of these materials.
Materials And Methods:
Three denture base materials-conventional heat-cured poly (methyl methacrylate) (PMMA), high-impact PMMA, and CAD/CAM-milled PMMA-were evaluated. RAW 264.7 macrophages were exposed to material eluates with and without STE. Cytokine levels were quantified using ELISA.
Results:
All materials showed significantly increased cytokine release in STE environments, with conventional PMMA eliciting the highest levels. CAD/CAM PMMA showed the least cytokine induction.
Conclusion:
Exposure to smokeless tobacco enhances the pro-inflammatory potential of denture base materials, with conventional PMMA being the most reactive. CAD/CAM PMMA demonstrates superior biocompatibility in inflammatory conditions.
