関連する実験動画

Updated: Jan 13, 2026

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.4K

致死的な植物遺伝子の低機能突然変異体作製のための開始コドン標的ゲノム編集戦略

Mika Yoshimura1, Tsubasa Mamiya2, Naoki Takahashi2

  • 1Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto, Kumamoto 860-8555, Japan.

Plant biotechnology (Tokyo, Japan)
|January 12, 2026
PubMed
まとめ

科学者たちは、開始コドンを除去することにより、部分的な遺伝子機能（低機能アレル）を作製するための新しいゲノム編集方法を開発した。このアプローチは、DNA修復とゲノム安定性に関する洞察を明らかにし、必須植物遺伝子の研究を支援する。

キーワード:
CRISPR-Cas9DNA損傷応答NSE1ゲノム編集低機能突然変異アレル

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関連する概念動画

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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