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Updated: Jan 14, 2026

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

絶食と再給餌の周期的な変化は、褐色脂肪組織における脂質の飽和、貯蔵、分布を再形成する

Xing Zhang^1,2,3,4, Ting Jiang^5,6, Chunqing Wang¹

¹Department of Biochemistry and Molecular Biology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, United States of America.

|January 12, 2026

Abstract:

Brown adipose tissue (BAT) functions as a metabolic sink, efficiently processing fatty acids (FAs), glucose, and amino acids, playing a pivotal role in metabolic regulation and energy homeostasis. However, the metabolic adaptations enabling BAT to respond to fasting and refeeding cycles are not well understood. Using mass spectrometry techniques-Liquid Chromatography (LC), Capillary Electrophoresis (CE), and Spatially Resolved Imaging-we demonstrate that BAT exhibits a unique free fatty acid (FFA) and lipid-bound FA profile, with enrichment of very long-chain polyunsaturated fatty acids (VLC-PUFAs) and C13-C14 FAs compared to white adipose tissue (WAT) in male C57BL/6 mice. Alternate-day fasting (ADF) triggered a dynamic change of these FFAs in BAT, accompanied by selective alterations of upper glycolysis, glyceroneogenesis, and triglyceride synthesis, a shift less pronounced in WAT. Additionally, several BAT lipid species, including glycerolipids, glycerophospholipids, and sphingolipids, transitioned from highly unsaturated to more saturated lipids upon refeeding, alongside significant spatial and dynamic reprogramming. Mechanistically, periodic fasting and refeeding activated mTORC1, and genetic inactivation of mTORC1 in BAT diminished ADF-induced lipid saturation, storage, and redistribution in the C57BL/6 background. These findings reveal that while BAT generally prefers unsaturated fats, it undergoes substantial lipid saturation and spatially dynamic reprogramming in response to fasting and refeeding, offering new insights into BAT's adaptive role in metabolic homeostasis.

キーワード:

褐色脂肪組織脂質代謝絶食再給餌 mTORC1 エネルギー恒常性脂肪酸脂質飽和度

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