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Updated: Jan 14, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

RALYをDSCR1転写の新規調節因子として同定

Jiwon Park¹, Chiyeol Choi², Hyerin Kim³

¹Department of Biomedical Sciences and Department of Physiology, Korea University College of Medicine, Seoul, 02841, South Korea; Department of Biological Sciences, College of Information and Biotechnology, Ulsan National Institute of Science and Technology, Ulsan, South Korea.

Biochemical and biophysical research communications

|January 12, 2026

Abstract:

Down syndrome critical region 1 (DSCR1) is located on chromosome 21, whose N-terminal coding sequence lacks recognizable motifs, and its precise transcriptional and translational regulation remains incompletely understood. Utilizing a bicistronic reporter system, we identified the core promoter region of DSCR1, which enables investigation of its gene regulatory mechanisms. Proteomic analyses via LC-MS/MS revealed that RALY directly binds to the DSCR1 promoter, influencing its transcriptional activity. Notably, RALY overexpression markedly suppresses DSCR1 expression, whereas siRNA-mediated knockdown of RALY does not significantly alter DSCR1 levels, suggesting complex regulatory dynamics. Given DSCR1's vital roles in Down syndrome (DS) phenotypes and critical biological processes such as neuronal development and immune regulation, modulation of RALY-mediated transcription may represent a novel mechanism for maintaining cellular homeostasis.

キーワード:

DSCR1 ダウン症候群 LC-MS/MS RALY

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CD Spectroscopy to Study DNA-Protein Interactions

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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

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