ストレスに弱く不安定なタンパク質のための添加剤ベースのタンパク質安定化

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Updated: Jan 14, 2026

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor

Published on: June 29, 2021

Tomoto Ura¹, Takumi Nakamura¹, Moe Iijima¹

¹Institute of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.

Protein expression and purification

|January 12, 2026

Abstract:

Protein-stabilizing additives have traditionally been investigated under dilute solution conditions using simple monomeric proteins as model systems. While such studies have clarified fundamental additive-protein interactions, their direct applicability to practical research and industrial contexts remains limited. This review leverages insights from model systems to summarize additive-based stabilization strategies in two major directions. First, we address process-related stresses, focusing on high-concentration conditions and interfacial stress frequently encountered during protein handling. Second, we highlight strategies for protein classes that are challenging to stabilize or purify, including membrane proteins, viral spike proteins, and intrinsically disordered proteins. This review aims to encourage additive use when proteins are difficult to stabilize under challenging conditions.

キーワード:

添加剤高濃度製剤プロセス関連ストレスタンパク質安定化不安定タンパク質

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How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

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