オンサイトユニバーサル植物マイコプラズマ検出のための23S rRNA遺伝子標的ループメディエテッドアイソサーマル増幅（LAMP）

This chapter describes a genus-universal, specific, sensitive, rapid, and simple detection system for phytoplasmas using universal loop-mediated isothermal amplification (LAMP) primers targeting the 23S rRNA gene. Diagnosis with this system consists of a simple boiling DNA extraction method (95 °C, 10 min) and DNA amplification by the LAMP assay (64 °C, 30-60 min), with a total time of about 60 min. This system enables on-site diagnosis, as it proceeds at a constant temperature, is easy to operate, requires no experimental devices or electricity, and generates little experimental waste. Moreover, the simple boiling DNA extraction method can be applied to a variety of sample types, including herbaceous plants, woody plants, and insect vectors.