ブドウの形質転換プロトコル：組織調製、プロトプラスト単離、および培養

Grapevine is among the most important economic plants that are susceptible to phytoplasma disease. Tools and procedures for genetic manipulation of grapevine are cumbersome and sometimes difficult to replicate; therefore, most of the research on plant-phytoplasma interactions focuses on species with more established transformation procedures instead, even though they might not be hosts of the studied phytoplasma. Studying the mechanisms of plant-phytoplasma interactions directly in the host plants would bring new and more accurate insights into the disease mechanisms. In this chapter, we present the protocols to obtain transformation-competent grapevine material from different tissues and the procedures for protoplast isolation and cultivation.