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関連する概念動画

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

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Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
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Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

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The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
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Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

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Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
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T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

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The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
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B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

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The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
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関連する実験動画

Updated: Jan 15, 2026

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
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反復刺激に対するナチュラルキラー細胞機能の動態

Jennifer One1, Janani Narayan2, Frank Cichocki3

  • 1Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.

Biotechnology progress
|January 14, 2026
PubMed
まとめ

がん治療のためのナチュラルキラー（NK）細胞の増殖は、K562フィーダー細胞による反復刺激によって行われる。しかし、この過程はNK細胞の増殖、代謝、機能に変化をもたらすため、効果的な細胞製品開発のためには注意深いモニタリングが必要である。

キーワード:
バイオ製造細胞傷害性増殖代謝ナチュラルキラー細胞

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Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
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Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
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関連する実験動画

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科学分野:

  • 免疫学
  • 細胞生物学
  • バイオテクノロジー

背景:

  • ナチュラルキラー（NK）細胞は、がん免疫療法において重要である。
  • 同種異系、すぐに使用できるNK細胞療法には、最大の細胞増殖が必要である。
  • 現在の増殖方法ではK562フィーダー細胞が使用されているが、NK細胞への影響は不明である。

研究 の 目的:

  • K562フィーダー細胞による反復刺激がNK細胞の増殖、代謝、機能に及ぼす影響を調査すること。
  • 大規模バイオ製造中のNK細胞の動態と細胞プロセスにおける変化を理解すること。

主な方法:

  • NK細胞とK562フィーダー細胞による連続刺激。
  • 細胞代謝を分析するためのSeahorse代謝フラックスアッセイ。
  • 遺伝子発現変化を評価するためのトランスクリプトミクス。
  • サイトカイン分泌と殺傷能力を評価するための機能アッセイ。

主要な成果:

  • 刺激後3〜4週頃にNK細胞の増殖動態と代謝に変化が生じた。
  • 代謝分析により、刺激初期には解糖系から酸化的代謝への移行が明らかになった。
  • 持続的な増殖にもかかわらず、増殖動態は全体的な代謝活性の低下と相関していた。
  • 長期間の刺激により、サイトカイン分泌と細胞傷害性機能の変化が観察された。

結論:

  • K562による連続刺激は、治療目的でNK細胞を効果的に増殖させる。
  • この増殖プロセスは、NK細胞の増殖、代謝、機能に大きな影響を与える。
  • 大規模NK細胞バイオ製造の最適化と製品の効果を保証するためには、徹底的な特性評価が不可欠である。