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関連する概念動画

CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

617
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
617
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.7K
CRISPR01:59

CRISPR

57.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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関連する実験動画

Updated: Jan 15, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
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Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

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CRISPR干渉によるサツガエラエ多遺伝子座配列型17株の研究

William D Cutts1, Aidan W Flanagan2, Brice K Gorman2

  • 1Department of Molecular and Cell Biology, University of Texas at Dallas, Dallas, Texas, USA.

Journal of bacteriology
|January 14, 2026
PubMed
まとめ

研究者らは、病原性連鎖球菌（GBS）の病原性を研究するために、CRISPR干渉（CRISPRi）システムを開発した。このツールは、特に病原性の高いST-17株COH1において標的遺伝子のノックダウンを可能にし、新生児髄膜炎の研究を助ける。

キーワード:
CRISPRB群レンサ球菌

さらに関連する動画

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

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関連する実験動画

Last Updated: Jan 15, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
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科学分野:

  • 微生物学
  • 遺伝学
  • 感染症

背景:

  • B群レンサ球菌（GBS）は、新生児細菌性髄膜炎の主な原因である。
  • 特に病原性の高いIII型血清型、配列型17（ST-17）株COH1は重症例と関連しているが、遺伝学的な操作が困難である。
  • 新生児髄膜炎の克服には、血液脳関門（BBB）におけるGBS病原性因子の理解が不可欠である。

研究 の 目的:

  • GBS ST-17 COH1株における標的遺伝子ノックダウンのための新規CRISPR干渉（CRISPRi）システムの開発。
  • GBS病原性因子の機能ゲノミクスおよびハイスループットスクリーニングの実現。
  • BBBにおけるGBS相互作用の調査。

主な方法:

  • 触媒不活性化Cas9（dCas9）を用いたCOH1株におけるCRISPR干渉（CRISPRi）システムの開発。
  • 溶血アッセイおよびqPCR転写解析によるシステム有効性の確認。
  • ヒト脳血管内皮細胞感染モデルを用いたin vitroでの遺伝子ノックダウン効果の評価。

主要な成果:

  • CRISPRiシステムは、ST-17 GBSにおいて調整可能な遺伝子発現ノックダウンを達成することに成功した。
  • 主要な病原性遺伝子（PI-2b、srr2、iagA）の表現型ノックダウンが観察された。
  • BBBにおける細菌の付着、侵入、および炎症反応の低下が実証された。

結論:

  • 開発されたCRISPRiプラットフォームは、ST-17 GBSにおける遺伝子操作のための汎用性の高いツールを提供する。
  • このシステムは、GBSの迅速な機能ゲノミクスおよび病原性研究を促進する。
  • 本研究結果は、GBSの病原性および潜在的な治療標的のより深い理解に貢献する。