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Updated: Jan 15, 2026

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells

Published on: June 3, 2012

インフルエンザAウイルスの細胞活性化タンパク質1 cFosはインフルエンザAウイルスの複製を調節する

Antoine Gerodez^1,2, François E Dufrasne¹, Olivier Denis³

¹National Influenza Center, Sciensano, 1180 Brussels, Belgium.

The Journal of general virology

|January 14, 2026

Abstract:

Previous research has demonstrated that influenza A virus (IAV) infection activates activating protein-1 (AP-1) transcription factors as part of the antiviral response. In this study, we identified cFos as the most upregulated AP-1 transcription factor during IAV infection in A549 human lung cells. Surprisingly, the knockdown of cFos resulted in impaired IAV replication. Fluorescence microscopy and functional analyses indicated that cFos is implicated in IAV infection through its nuclear function, rather than its cytoplasmic role as an activator of lipid synthesis. The investigation into the role of cFos in IAV infection revealed increased apoptosis and elevated interferon-β mRNA levels in cFos-knockdown A549 cells during IAV infection. This suggests that cFos may enhance cell survival and reduce interferon-β expression during infection, thereby facilitating IAV proliferation. Furthermore, the levels of viral NA mRNA and the expression of late viral proteins NA and M2 decreased upon cFos-knockdown. Overall, this study identifies cFos as a proviral factor for IAV, through the modulation of innate immunity and apoptosis during infection, and potentially by supporting the viral transcription.

キーワード:

AP-1 アポトーシス cFos インフルエンザAウイルスインターフェロン転写因子

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The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

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Published on: April 25, 2016

Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection

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Published on: July 21, 2022

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