ナノポアを用いたアミノ酸一文字分解能による全長タンパク質の単一分子同定

Andrea Bonini, Chunzhe Lu, Luca Mantovanelli

bioRxiv : the preprint server for biology

|January 16, 2026

Abstract:

Nanopore-based technologies show promise in single-molecule protein sequencing. By using an unfoldase and a nanopore with enhanced electroosmotic flow, here we show the continuous identification of generic proteins during single nanopore passes. This approach enables the recording of differences in charge and size from single amino acid substitutions compared to reference signals, paving the way for single-molecule protein sequencing and high-throughput proteomics.

キーワード:

ナノポアタンパク質シーケンシングプロテオミクス単一分子アミノ酸置換

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