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Updated: Jan 18, 2026

Detection of Anti-MDA5 Autoantibodies Using HeLa Cells and Immunocytochemistry with Light Microscopy

Detection of Anti-MDA5 Autoantibodies Using HeLa Cells and Immunocytochemistry with Light Microscopy

Published on: October 31, 2025

間質性肺疾患を伴う抗核抗体陰性皮膚筋炎の1例

Ying Qian, Kejie Xie

Clinical laboratory

|January 16, 2026

要旨

Background:

Antinuclear antibody (ANA) is important for the diagnosis of autoimmune diseases. When ANA is positive, further specific autoantibody tests are needed to make a definite diagnosis.

Methods:

This article reports a case of a patient with an autoimmune disease who had inconsistent results in the detection of antinuclear antibodies by indirect immunofluorescence assay (IIFA) and linear immunoblotting assay (LIA).

Results:

This patient presented with negative ANA and positive anti-SSA/Ro52.

Conclusions:

IIF-ANA negative and LIA-ANAS positive exists in clinical tests. The combination of IIFA and LIA is important.

キーワード:

抗核抗体陰性皮膚筋炎間質性肺疾患自己免疫疾患診断間接蛍光抗体法線状イムノブロットアッセイ

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Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Published on: June 16, 2020

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Published on: June 23, 2014

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