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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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ベンチトップNMR技術を用いたチップ上代謝解析

Marc Azagra1, Hetal Patel2, Alejandro Portela1

  • 1Institute for Bioengineering of Catalonia, Barcelona Institute of Science and Technology, 08028 Barcelona, Spain.

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まとめ

我々は、臓器チップシステムのリアルタイム代謝モニタリングのために、新規ベンチトップ核磁気共鳴（NMR）分光計を開発しました。この画期的な技術により、マイクロ流体プラットフォーム内での非侵襲的な細胞代謝解析が可能になります。

キーワード:
チップ上解析核磁気共鳴代謝モニタリング臓器チップ過分極

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科学分野:

  • バイオテクノロジー
  • 分析化学
  • 細胞生物学

背景:

  • 臓器チップ（OoC）システムは高度なinvitroモデルですが、リアルタイムで非侵襲的な代謝モニタリングツールが不足しています。
  • 核磁気共鳴（NMR）分光法は強力な代謝解析技術ですが、現在のセットアップはマイクロ流体チップ形式との互換性がありません。

研究 の 目的:

  • マイクロ流体プラットフォーム上の細胞培養のリアルタイム代謝モニタリングのために設計された初のベンチトップNMR分光計を導入すること。
  • 既存のNMR技術を臓器チップシステムとのシームレスな統合に適応させること。

主な方法:

  • 過分極（溶解動的核分極、dDNP）を利用してNMR信号感度を向上させました。
  • 市販のベンチトップNMR分光計を改造し、精密な基質注入と培地更新のためのカスタムマイクロ流体プラットフォームを設計しました。
  • 信号送受信のために高周波コイルとカスタムサンプルキャリアを統合しました。

主要な成果:

  • 開発されたシステムを用いたマイクロ流体プラットフォーム上でのリアルタイム代謝モニタリングの実現可能性を実証しました。
  • 統合されたセットアップ内での動的な代謝研究を示す予備的なNMRデータを取得しました。

結論:

  • 開発されたベンチトップNMR分光計は、臓器チップ研究における非侵襲的な代謝解析のための重要な進歩を表しています。
  • この技術は、マイクロ流体細胞培養モデルの生理学的関連性と分析能力を高める動的な代謝研究を促進します。