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Updated: Jan 22, 2026

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

出芽酵母におけるAtg2-Atg18複合体とAtg1複合体の相互作用による前オートファゴソーム構造への局在化

Yuri Yasuda^1,2, Kanae Hitomi², Nobuo N Noda^3,4

¹Cell Biology Center, Institute of Integrated Research, Institute of Science Tokyo, Yokohama, Japan.

Molecular biology of the cell

|January 21, 2026

Abstract:

During autophagy induction in Saccharomyces cerevisiae, over twenty autophagy-related (Atg) proteins localize to the site of autophagosome formation to generate the pre-autophagosomal structure (PAS), where phase-separated condensates of the Atg1 kinase complex serve as a scaffold for recruiting other Atg proteins. The lipid transfer protein Atg2 forms a complex with the phosphatidylinositol 3-phosphate (PI3P)-binding protein Atg18 and mediates lipid influx from the endoplasmic reticulum to the PAS for membrane expansion. In this study, we discover that the Atg2-Atg18 complex interacts with the Atg1 complex. This interaction involves the C-terminal regions of Atg2 and the Atg1 complex subunit Atg29, and is enhanced by Atg1-dependent phosphorylation of Atg29. This interaction, together with Atg18 binding to PI3P, promotes PAS localization of the Atg2-Atg18 complex. These findings provide new insight into PAS organization and highlight the Atg1 complex as a central hub coordinating Atg protein assembly during autophagosome formation.

キーワード:

オートファジー Atg1複合体 Atg2-Atg18複合体前オートファゴソーム構造 PAS 出芽酵母タンパク質相互作用リン酸化

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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

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