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Updated: Jan 23, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

植物における飢餓状態でのBZR1相互作用体をメタボリック安定同位体標識定量IP-MSにより同定するためのプロトコル

Zhenzhen Zhang¹, Zhi-Yong Wang²

¹Synthetic Biology Center, Haixia Institute of Science and Technology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

|January 21, 2026

Abstract:

Identification of interacting proteins, particularly those mediating protein degradation, remains technically challenging. The transcription factor BZR1 in Arabidopsis is activated by brassinosteroid (BR) signaling to promote plant growth and is degraded under starvation conditions to restrict growth and promote survival. Here, we present a protocol for identifying BZR1 interactors in plants under sugar starvation using ¹⁵N stable isotope labeling (SIL) followed by immunoprecipitation and quantitative mass spectrometry (IP-MS) analysis. This protocol has broad potential applications in studying the protein interactome. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.

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植物科学プロテオミクスシグナル伝達

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Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

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A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

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