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Updated: Jan 23, 2026

Preparation of Binary and Ternary Deep Eutectic Systems

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

エタノールと深共晶溶媒を用いたジャンボラン果実からのアントシアニンの抽出：生物活性、細胞毒性、および天然着色料としての応用

Pedro Victor Crescêncio de Freitas¹, Carlos Eduardo de Araújo Padilha², Marcos Dos Santos Lima³

¹Biotechnology Postgraduate Program - RENORBIO, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.

|January 21, 2026

Abstract:

This study aimed to evaluate the extraction of anthocyanins from jambolan (Syzygium cumini) fruit using choline chloride-based deep eutectic solvents (DESs) as co-solvents in ethanolic systems. The extraction was performed using ethanol and choline chloride-based DESs with xylitol (ChX-DES) and glycerol (ChG-DES). The highest anthocyanin extraction was achieved using ethanol with 5% DES, resulting in 20.40 to 23.36 mg M3G/ 100 g. Increased temperature favored the extraction, particularly at 70 °C for 5% ChG-DES, yielding 28.92 mg M3G/100 g. The ethanol containing 5% ChG-DES extract exhibited higher color intensity, suggesting its potential as a natural colorant. Additionally, DES-containing extracts demonstrated higher antioxidant capacity and α-amylase and amyloglucosidase inhibition. Cytotoxicity in the HaCaT cell revealed no toxicity up to 12.5 mg/mL. Finally, the color stability of yogurts enriched with the ChG-DES extract was maintained during storage, highlighting the potential of DESs for extracting and applying anthocyanins as natural colorants and functional agents in foods.

キーワード:

ジャンボランアントシアニン深共晶溶媒天然着色料抽出抗酸化作用食品化学

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