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Updated: Jan 23, 2026

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization

Published on: August 11, 2018

天然由来の抗菌補助剤によるプラスミド媒介性細菌耐性の逆転

Linyu Wu¹, Xinyuan Zhang¹, Peter Oelschlaeger²

¹Department of Forestry Chemical Engineering, Shaanxi Key Laboratory of Economic Plant Resources Development and Utilization, Forestry College, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.

Journal of agricultural and food chemistry

|January 21, 2026

Abstract:

The clinical efficacy of β-lactams, polymyxins, and tetracyclines is especially increasingly compromised by plasmid-mediated resistance mechanisms. These include but are not limited to expression of β-lactamases (BLs), MCRs (encoded by the mcrs colistin resistance gene), and TetX (encoded by the tet(X3)/tet(X4) tetracycline resistance gene), which have constituted a significant menace to public health and food safety, triggering urgent concern worldwide. Combating this crisis requires innovative approaches, among which the development of antimicrobial adjuvants that synergize with existing antibiotics holds particular promise for reclaiming lost therapeutic efficacy. This review systematically summarizes the plasmid-mediated resistance mechanisms that inactivate β-lactams, polymyxins, and tetracyclines. Furthermore, we provide a detailed overview of all identified natural product chemotypes capable of inhibiting SBLs, MBLs, MCRs, and Tet(X3)/Tet(X4) enzymes or functioning as antimicrobial adjuvants along with their mechanisms of action. These findings establish fundamental principles to guide the development of broad-spectrum antimicrobial adjuvants aimed at counteracting plasmid-mediated bacterial resistance.

キーワード:

抗菌補助剤細菌耐性天然物プラスミド媒介性耐性 β-ラクタマーゼ阻害剤

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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

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Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

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