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Updated: Jan 23, 2026

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

Published on: May 13, 2020

空気中呼吸器微生物叢の定量化による空気感染リスクの解明

Henry P Oswin¹, Raymond Tellier², Robert Groth¹

¹Queensland University of Technology, International Laboratory for Air Quality and Health, Brisbane, Australia.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

|January 21, 2026

要旨

Objective:

To develop airborne respiratory microflora quantification as a method of quantifying airborne infection risk that is more readily applicable than airborne pathogen sampling, without being subject to the limitations and caveats of CO2 monitoring.

Methods:

Digital PCR targeting a mixture of Streptococcus and Haemophilus genera common to the respiratory tract was first tested under laboratory conditions and then tested on aerosol sampled from around a hospital in Sydney, with PCR quantification of SARS-CoV-2 genome copies also being carried out on the samples.

Results:

Clear signals were obtained from every location within the hospital, with a significantly higher signal being observed in more densely crowded, less well-ventilated areas. When SARS-CoV-2 was present within the aerosol samples, the respiratory microflora signal correlated with the number of SARS-CoV-2 copies.

Conclusions:

Airborne respiratory microflora can be used as a marker for airborne infection risk. Using the value in conjunction with pathogen sampling provides in-depth insights into the relative infection risk of a space, as well as a clear marker which can be used to compare between different pathogen sampling studies.

キーワード:

Aerobiology Disease transmission Infection risk quantification SARS-CoV-2

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Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

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Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface

Published on: February 23, 2020

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