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Updated: Jan 24, 2026

A β-glucuronidase GUS Based Cell Death Assay

A β-glucuronidase GUS Based Cell Death Assay

Published on: May 6, 2011

PtBGL：植物インプリント染色に応用可能な、コスト効率の良いGUSレポーター代替法

Yuyao Su¹, Yitong Li¹, Jiali Zhong¹

¹Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, School of Life Sciences, Guangzhou University, Guangzhou, China.

Frontiers in plant science

|January 23, 2026

Abstract:

Reporter gene systems are essential tools for monitoring gene expression and transformation efficiency in plant research. The widely used GUS system provides strong, stable, and well-localized signals but requires expensive substrates. Here, we present an alternative based on Persicaria tinctoria β-glucosidase (PtBGL) and its natural substrate indican, which is a low-cost indoxyl glucoside extractable from heat-treated indigo plants. When expressed in Arabidopsis thaliana and Nicotiana benthamiana, PtBGL localizes to plastids and converts indican into a blue indigo pigment visible to the naked eye. Comparative assays in 13 of the 14 tested plant species revealed no detectable background activity from endogenous β-glucosidases at the examined developmental stage. The PtBGL and indican system enables cost-effective transgenic screening, gene expression analysis, and practical applications such as plant-based fabric imprinting.

キーワード:

PtBGL 安価な基質遺伝子発現インプリント染色レポーター遺伝子

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