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Updated: Jan 24, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

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シアログリカン結合性ビリダンズ連鎖球菌が、既知の結合部位を超えるサイズのリガンドをどのように収容するかの構造的根拠

KeAndreya M Morrison1, Kole Martin2, Hai Yu3

  • 1Department of Pharmacology, Meharry Medical College, Nashville, TN, US.

microPublication biology
|January 23, 2026
PubMed

心内膜感染において、ビリダン Streptococcus 群は、siglec様結合領域を含むタンパク質を用いて、シアル酸でキャップされたO-血小板糖タンパク質GPIbα上のGalNAc糖鎖について。これまでの多くの研究では、このシアロ糖鎖結合を解析するために、単離された二糖、三糖、または四糖の部分リガンドが用いられてきました。本報では、1.9 Å分解能の結晶構造を報告します。Streptococcus gordoniiL-セリン結合シアル酸T抗原（sTa）三糖に結合したstrain M99 siglec様結合領域。この構造は、三糖の伸長がどのように収容されるかを示しており、より大きなシアログリカンリガンドとの結合への影響を示唆している。

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Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Ligand Binding Sites02:40

Ligand Binding Sites

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The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

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