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シャガス病感受性と慢性シャガス心筋症重症度に関連する循環マイクロRNAのトランスクリプトーム解析

Eric Henrique Roma¹, Fabielle Marques-Santos^1,2, Alinne Rangel Dos Santos Renzetti^1,2

¹Laboratório de Imunologia e Imunogenética em Doenças Infeciosas - Instituto Nacional de Infectologia Evandro Chagas - Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.

The Journal of infectious diseases

|January 23, 2026

要旨

Background:

Chagas disease (ChD), a major public health concern in Latin America, requires better understanding of molecular mechanisms driving disease progression and identifying biomarkers.

Objective:

We investigated circulating microRNAs associated with ChD susceptibility and chronic Chagas cardiomyopathy (CCC) progression.

Methods:

In a cross-sectional study with 150 ChD patients (46 indeterminate, 104 CCC) and 42 non-ChD controls, we performed microRNA sequencing, and differentiated expressed microRNAs (DEMs) were submitted to in silico functional analyses.

Results:

We identified 40 DEMs between ChD patients and controls, highlighting upregulated miR-143-3p and miR-223-3p, and downregulated miR-486-5p and miR-3960 in ChD patients. These miRNAs showed involvement in immune response and cardiovascular signaling pathways. MiR-6734-5p, miR-1285-5p, miR-10527-5p and miR-1228-5p tended to be upregulated, while miR-30c-3p tended to be downregulated in severe compared to mild CCC patients.

Conclusion:

This study provides evidence for specific microRNA dysregulation in ChD, highlighting their potential as biomarkers of ChD susceptibility and CCC severity.

キーワード:

シャガス病心筋症病気の進行マイクロRNA トランスクリプトーム

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