子宮頸がんのトランスレーショナルリサーチをバイオバンキングを通じて推進するためのプロトコル：バージニアコモンウェルス大学子宮頸部細胞診バイオリポジトリ

Cervical cancer remains a major global health concern, with disparities in screening access and outcomes, particularly among younger women in the United States. Although high-risk HPV infection is the primary etiologic factor, non-genetic and environmental contributors remain underexplored. We established the Virginia Commonwealth University (VCU) Cervical Cytology Biorepository to support research on cervical cancer risk, molecular biomarkers, and gynecologic health disparities.

We implemented standardized protocols for recovery, processing, and long-term storage of residual ThinPrep Pap specimens from women aged 21-65 receiving routine gynecologic care across VCU Health outpatient clinics. Core elements included an opt-out consent model, an IRB-approved waiver for discarded cytology material, EHR linkage for longitudinal clinical data, and workflows for specimen recovery, aliquoting, and processing of pellet and supernatant fractions. Specimens are cryostored at -80 °C within the Goodwin Research Laboratory with quality control checks and secure data governance.

The biorepository supports standardized recovery and processing of residual Pap specimens, yielding sufficient post-diagnostic volume and enabling high-quality genomic DNA extraction and metabolomic profiling. Pilot genomic and metabolomic data demonstrate that residual cervical cytology material provides adequate input for multi-omic analyses, confirming feasibility and scalability.

This protocol outlines a framework for establishing a cervical cytology biobank using residual clinical specimens. The integration of biospecimens with linked clinical and demographic data enables multidimensional research into cervical cancer etiologies and other gynecologic conditions.

This resource supports molecular epidemiology studies aimed at identifying biomarkers, understanding cervical cancer progression, and addressing reproductive health disparities in diverse populations.