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Proteomics01:33

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
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ATP Driven Pumps II: P-type Pumps01:34

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The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
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V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
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プロテオミクスによる大腸がんの硬さ駆動型変化の解読

Charlotte Cresens1, Ana Montero-Calle2, Guillermo Solís-Fernández3

  • 1Molecular Imaging and Photonics Division, Chemistry Department, Faculty of Sciences, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium.

Molecular & cellular proteomics : MCP
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PubMed
まとめ

腫瘍の硬化は、大腸がん細胞の分泌プロテオームを著しく変化させ、移動と血管新生を促進する。これは基質硬度を強調する

キーワード:
腫瘍の硬さ大腸がん機能的アッセイ細胞内および分泌プロテオームプロテオミクス分泌プロテオーム

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科学分野:

  • 生物医学工学
  • がん生物学
  • プロテオミクス

背景:

  • 腫瘍の硬化は、がんの進行と転移における重要な要因である。
  • 腫瘍微小環境の機械的特性は、がん細胞の挙動に影響を与える。
  • 腫瘍の硬度がタンパク質発現に及ぼす影響に関する研究は限られている。

研究 の 目的:

  • 基質硬度が大腸がんにおけるタンパク質異常調節に及ぼす影響を調査すること。
  • 変化した基質硬度に応答する特定のタンパク質変化を同定すること。
  • これらのタンパク質変化ががんの進行にどのように影響するかを理解すること。

主な方法:

  • 大腸がん細胞の綿密なプロテオミクス解析。
  • 様々な基質硬度条件下でのタンパク質発現の比較。
  • 細胞移動、血管新生、および基質リモデリングを評価するための機能的アッセイ。

主要な成果:

  • 基質硬度は、分泌タンパク質（分泌プロテオーム）の発現を著しく変化させた。
  • 細胞内タンパク質レベルは、基質硬度の変化によってほとんど影響を受けなかった。
  • 硬度誘発性の分泌プロテオームの変化は、細胞移動、血管新生、および基質リモデリングを促進した。

結論:

  • 基質硬度は、分泌プロテオームの変化を介して大腸がんの進行を駆動する上で重要な役割を果たしている。
  • 分泌プロテオームの変化は、より攻撃的ながん表現型に寄与する。
  • 本研究結果は、新規の生体力学的がん治療法の開発に洞察を提供する。