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Updated: Jan 28, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

ニューモニア菌における新規蛍光法を用いた迅速コリスチン感受性決定

Meliha Negin^1,2, Meltem Ayas^3,4, Isin Akyar⁴

¹Department of Medical Biotechnology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul, Türkiye.

Frontiers in microbiology

|January 26, 2026

要旨

Introduction:

Klebsiella pneumoniae is a causative agent that can lead to fatal infections, especially in intensive care patients. Colistin treatment is employed as a last resort in K. pneumoniae infections caused by strains resistant to almost all antibiotics from different groups. However, in recent years, colistin resistance has also become widespread in K. pneumoniae strains. Therefore, before starting treatment with this relatively toxic drug, it is essential to know its susceptibility to colistin. A standard colistin susceptibility test typically takes 24-48 h. In this study, we investigated the accuracy of our novel fluorometric method, which enables the determination of colistin minimal inhibitory concentration (MIC) within 2 h for K. pneumoniae.

Methods:

For this purpose, we determined and compared the MIC values of 24 colistin-resistant and 18 colistin-susceptible K. pneumoniae strains using the standard broth microdilution method and the new fluorometric method.

Results:

In the comparison of MIC values determined by standard and fluorometric methods, it was observed that the MIC values of 22 strains were identical, there was a 1-dilution (1/2) difference in 14 strains, and a 2-dilution (1/4) difference in six strains.

Discussion:

We believe that the new fluorometric antibiotic susceptibility testing method will be a valuable tool in the rapid detection of colistin resistance in K. pneumoniae and thus for guiding appropriate treatment in critically ill patients.

キーワード:

肺炎桿菌コリスチン耐性蛍光アッセイ最小発育阻止濃度迅速薬剤感受性試験

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