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Updated: Jan 29, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

コンピュータ解析と生物学的スクリーニングの収束：超高感度オキシフルオルフェン検出のための高度なイムノアッセイプラットフォーム

Huihui Wu¹, Lingling Guo¹, Xinxin Xu¹

¹International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

|January 27, 2026

Abstract:

The common use of Oxyfluorfen (Oxy), a diphenyl ether herbicide, in agriculture, necessitates the development of rapid detection methods to ensure food safety, mitigate environmental risks, and enable on-site pesticide residue monitoring. In this study we developed a highly sensitive immunochromatographic strip for the detection of Oxy. Through computer-assisted hapten design, antibody screening, and system optimization, the method achieved rapid detection of Oxy residues in agricultural products. The optimized strip demonstrated calculated limits of detection (cLOD) of 9.96-14.71 ng/g in apples, cucumbers, cowpeas, and lettuce, significantly lower than the national standard limit. Method validation showed spike recovery rates of 95.2-98.8%, with high consistency to LC-MS/MS results (R² > 0.99). This technology offers advantages such as simplicity (results in 8 min), low cost, and resistance to matrix interference, making it suitable for on-site monitoring of agricultural product safety.

キーワード:

コンピュータ支援イムノクロマトグラフィー現場モニタリングオキシフルオルフェン迅速検出

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