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関連する概念動画

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Sequences01:29

Sequences

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Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Arithmetic Sequences01:30

Arithmetic Sequences

234
An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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3' End Sequencing Library Preparation with A-seq2
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SCITO-seq2: 超ハイスループット単一細胞トランスクリプトームおよびエピトープシーケンシング

Su-Hyeon Lee1, Bo-Yeong Jin2,3, Cho-Rong Lee4

  • 1Department of Biomedical Sciences, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.

Genome biology
|January 28, 2026
PubMed
まとめ
この要約は機械生成です。

SCITO-seq2は、10万以上の細胞でRNAおよびタンパク質プロファイリングを組み合わせることで、単一細胞マルチオミクスを前進させます。この費用対効果の高いプラットフォームは、免疫細胞のシグネチャを特定することにより、自己免疫疾患研究を強化します。

キーワード:
CTLA4ハプロ不全症と自己免疫浸潤小児全身性エリテマトーデス単一細胞マルチオミクス超ハイスループットシーケンシング

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Transcriptome Analysis of Single Cells
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関連する実験動画

Last Updated: Jan 29, 2026

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科学分野:

  • 免疫学
  • ゲノミクス
  • プロテオミクス

背景:

  • 単一細胞マルチオミクス技術は、複雑な生物学的システムを理解するために不可欠です。
  • 既存の方法は、スケーラビリティ、費用対効果、および正確な分子プロファイルのマッチングにおいて課題に直面しています。

研究 の 目的:

  • 超ハイスループット単一細胞RNAおよびタンパク質分析のための改良プラットフォームであるSCITO-seq2を導入すること。
  • 自己免疫疾患における免疫細胞の不均一性を解明する上でのSCITO-seq2の有用性を実証すること。

主な方法:

  • SCITO-seq2は、プローブベースのRNA検出と共有プールバーコーディングを使用したタンパク質プロファイリングを統合します。
  • このプラットフォームは、効率的なサンプルマルチプレキシングのためのセルハッシングをサポートします。
  • 自己免疫疾患コホートからの10万以上の細胞で分析が実行されました。

主要な成果:

  • SCITO-seq2は、大規模な細胞集団全体で転写産物と表面タンパク質の堅牢な定量化を達成しました。
  • マルチプレックス化されたドロップレット内の分子プロファイルの正確な照合は、バーコーディング戦略によって保証されました。
  • このプラットフォームは、自己免疫疾患におけるマイナーな免疫クラスターと疾患特異的なタンパク質シグネチャを特定することに成功しました。

結論:

  • SCITO-seq2は、スケーラブルで合理化された費用対効果の高い次世代単一細胞マルチオミクスワークフローを提供します。
  • この技術は、自己免疫疾患の根底にある細胞メカニズムのより深い洞察を可能にします。
  • SCITO-seq2は、新規バイオマーカーおよび治療標的の発見を促進します。