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Updated: Jan 29, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

油田生産プラットフォームからの水圧入システムにおける硫化物生成と微生物動態

Vitória da Silva Pereira Domingues¹, Maira Paula de Sousa², Vinicius Waldow²

¹Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro 21941-902, Brazil.

|January 28, 2026

Abstract:

In addition to seawater in the injection header (IH) to enhance oil recovery, oil companies reuse produced water (PW), a byproduct of oil extraction, and implement produced water reinjection systems (PWRI). Although the microorganisms in IH are controlled by biocides, PW is generally treated by flotation to remove oil residues before PWRI. However, IH, PW, and PWRI can be sources of sulfate-reducing bacteria (SRB) related to oil reservoir souring. Here, we evaluated hydrogen sulfide (H₂S) production in IH, PW, and PWRI, as well as the microbial dynamics (most probable number-MPN, quantitative PCR, and amplicon sequencing), of a Brazilian oil reservoir. Results revealed that the highest average H₂S concentration occurred in PW samples. However, the dissolved H₂S threshold concentration of 2 mg L^-1 was exceeded in 18% of PW and ~16% of PWRI samples, respectively. Although MPN showed no correlation between H₂S and the number of SRB or total anaerobic heterotrophic bacteria (TAHB), qPCR and microbiome data revealed that the SRB Desulfobacterota was the most abundant in PW and PWRI. Overall, flotation was associated with (i) low microbial control in PW; and (ii) the enrichment of SRB (mainly Desulfobacterota), Thermotogota, and Proteobacteria groups in PWRI.

キーワード:

石油貯留層生産水圧入二次石油回収硫酸塩還元菌水圧入

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