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Updated: Jan 31, 2026

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Association-induced folding governs surrogate light chain and pre-B cell receptor core assembly

Jasmin König^1,2, Natalia Catalina Sarmiento Alam^1,2,3, Ruiming He^1,2

¹Department Bioscience, School of Natural Sciences, Technical University Munich, Garching, Germany.

Nature communications

|January 29, 2026

まとめ

The surrogate light chain (SLC) component, λ5, is crucial for pre-B cell receptor (preBCR) assembly by inducing folding in VpreB and the heavy chain (HC). This process ensures proper preBCR structure and function during B cell development.

科学分野:

Immunology
Molecular Biology
Cell Biology

背景:

Pre-B cell receptor (preBCR) assembly is a critical checkpoint in B cell development.
Defective heavy chains (HC) necessitate robust quality control mechanisms.
The surrogate light chain (SLC), a VpreB/λ5 heterodimer, interacts with HC to form the preBCR.

研究の目的:

To elucidate the molecular mechanisms governing preBCR assembly.
To investigate the role of λ5 in SLC and preBCR formation.
To understand the structural and functional contributions of SLC components.

主な方法:

In vitro reconstitution of the preBCR.
Cell-based experiments to study preBCR assembly.
Analysis of protein folding and complex formation.

主要な成果:

λ5 is essential for preBCR assembly, inducing structure in VpreB and promoting HC C_H1 domain folding.
Association of λ5 with HC facilitates proper folding and endoplasmic reticulum (ER) release.
The unique region of λ5 is critical for antigen interaction within the SLC-HC complex.

結論:

PreBCR assembly involves a multi-step mechanism driven by association-induced folding.
λ5 acts as a key regulator of structural integrity and function during preBCR formation.
Understanding these mechanisms provides insights into B cell development and quality control.

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