Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Plant Tissue Culture02:57

Plant Tissue Culture

40.6K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
40.6K
Plant Cells and Tissues02:01

Plant Cells and Tissues

65.7K
Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
65.7K
Plant Tissues01:18

Plant Tissues

9.0K
Plants are multicellular eukaryotes with tissue systems made of various cell types that carry out specific functions. Different tissues work together to perform a unique function and form an organ. Organs working together form organ systems. Vascular plants have two distinct organ systems: a shoot system and a root system. The shoot system consists of two portions: the vegetative (non-reproductive) parts of the plant, such as the leaves and the stems, and the reproductive parts of the plant,...
9.0K
Tonicity in Plants00:53

Tonicity in Plants

59.8K
Tonicity describes the capacity of a cell to lose or gain water. It depends on the quantity of solute that does not penetrate the membrane. Tonicity delimits the magnitude and direction of osmosis and results in three possible scenarios that alter the volume of a cell: hypertonicity, hypotonicity, and isotonicity. Due to differences in structure and physiology, tonicity of plant cells is different from that of animal cells in some scenarios.
59.8K
Subcellular Fractionation01:32

Subcellular Fractionation

8.9K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
8.9K
Meristems and Plant Growth02:36

Meristems and Plant Growth

49.5K
Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
49.5K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Mild hypothermic pretreatment alleviates hepatic ischemia-reperfusion injury by reducing CIRP-dependent NETosis-related hepatocyte pyroptosis.

Cellular signalling·2026
Same author

San Wei Tan Xiang (SWTX) ameliorates depressive-like behaviors by modulating 6-phosphogluconate dehydrogenase (6PGD) activity in mice.

Journal of ethnopharmacology·2026
Same author

Single-Cell Transcriptome-Wide Mendelian Randomization and Colocalization Uncover Potential Immunocytes-Related Therapeutic Targets for Obesity.

Journal of cellular and molecular medicine·2026
Same author

Multi-target fluorescence staining of bacteria smears enables rapid machine learning-assisted species classification.

mLife·2026
Same author

Entanglement Superactivation in Multiphoton Distillation Networks.

Physical review letters·2026
Same author

Cortical regulation of collective social dynamics during environmental challenge.

Nature neuroscience·2026

関連する実験動画

Updated: Jan 31, 2026

Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria
10:35

Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria

Published on: October 6, 2022

3.4K

植物組織における細胞内スクロース濃度の解明

Xingjian Zhang1, Sandro Heschl1, Johannes Liesche1

  • 1Institute of Biology, University of Graz, 8010 Graz, Austria.

Journal of experimental botany
|January 30, 2026
PubMed
まとめ

植物細胞内のスクロース濃度を細胞レベルで測定することは困難であり、特に細胞質ゾルでは困難です。このレビューでは、植物スクロース測定のための様々な方法をカバーしており、将来の研究に役立ちます。

科学分野:

  • 植物生物学
  • 生化学
  • 細胞生物学

背景:

  • スクロースは植物における主要な炭素およびエネルギー源であり、光合成中に生成される。
  • 細胞内スクロースレベルは、植物の成長に不可欠な輸送および酵素反応を調節する。
  • 細胞質ゾルという薄い細胞層におけるスクロースの正確な測定は困難である。

研究 の 目的:

  • 植物細胞における細胞内スクロース濃度測定のための既存の方法論をレビューすること。
  • 各方法の応用と限界を議論すること。
  • 生体組織の糖濃度分析における進歩を強調すること。

主な方法:

  • マイクロオートラジオグラフィー
  • 非水性分画
  • フーリエ変換赤外(FTIR)顕微鏡法
  • ラマン顕微鏡法
  • 質量分析イメージング
  • FRETナノセンサー
  • 直接サンプリング
  • 理論モデリング

主要な成果:

キーワード:
炭素配分炭素代謝生細胞イメージング師管ソースシンクスクロース輸送

さらに関連する動画

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
08:39

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Published on: December 24, 2017

7.5K
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
10:13

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Published on: November 12, 2016

10.3K

関連する実験動画

Last Updated: Jan 31, 2026

Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria
10:35

Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria

Published on: October 6, 2022

3.4K
Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
08:39

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Published on: December 24, 2017

7.5K
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
10:13

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Published on: November 12, 2016

10.3K
  • 細胞内スクロース定量のための多様な技術の包括的な概要が提示されている。
  • 各方法の研究課題への適合性が議論されている。
  • 生細胞スクロース測定の現在の限界と将来の方向性が概説されている。

結論:

  • 植物生理学の理解には、正確な細胞内スクロース測定が不可欠である。
  • 植物スクロース分析には、それぞれ独自の強みを持つ様々な技術が存在する。
  • 植物におけるリアルタイムの生体内細胞内糖定量には、さらなる開発が必要である。