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Updated: Jan 31, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

核内 survivin と PRC2 複合体の相互作用、および H3K27Me3 指向性転写抑制への影響

Adesh D Vaidya¹, Alexander J Fezovich¹, Sally P Wheatley¹

¹University of Nottingham, Nottingham, UK.

Journal of cell science

|January 30, 2026

Abstract:

The PRC2 complex tri-methylates histone 3 at lysine 27 (k27), a post translational modification that induces heterochromatin formation and transcriptional repression. Survivin is a nucleocytoplasmic shuttling protein that is kept out of the nucleus in clement conditions, but that accumulates there in times of stress and in certain specialised cells. While the cytoplasmic functions of survivin are well documented, there is comparatively less understanding of its roles within the nucleus. Here we investigated whether nuclear survivin can affect transcriptional programming. Using interaction analyses and qPCR we report that it binds to the enzymatic subunit of the polycomb repressor complex 2, EZH2 and H3k27Me3, and causes depression of its target genes in a variety of human cells.

キーワード:

H3K27Me3 低酸素症ポリコーム抑制複合体 2 幹細胞 Survivin 転写

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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

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