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Updated: Feb 1, 2026
06:05
An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model
Published on: March 9, 2022
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乏精子症精子はミトコンドリア機能不全を呈し、DKK3欠損が病因因子である
Ruizhi Xue1, Longlong Zhang1, Ruikang Shi2
1Department of Urology and Andrology, Xijing Hospital of the Air Force Medical University, Xi'an, 710032, China.
Reproduction (Cambridge, England)
|January 30, 2026
まとめ
乏精子症（AS）は、男性不妊の主な原因であり、精子のエネルギー産生障害と酸化ストレスの上昇を伴います。Dickkopf（DKK）3の欠損は、ミトコンドリア機能と精子運動性の障害を通じてASに寄与します。
科学分野:
- Reproductive Biology; Mitochondrial Metabolism; Male Infertility
背景:
- Asthenozoospermia (AS) is a major cause of male infertility, but its metabolic underpinnings are not fully understood.; Understanding sperm metabolic profiles is crucial for diagnosing and treating AS.
研究 の 目的:
- To define the energy metabolic profile of AS spermatozoa.; To identify key proteins linked to metabolic dysregulations in AS.; To investigate the role of Dickkopf (DKK)3 in AS pathogenesis.
主な方法:
- Comparative analysis of sperm energy metabolism (ATP production, ETC activity, ROS, MMP) between AS patients and controls.; Proteomic analysis to identify differentially expressed proteins (DEPs) in AS spermatozoa.; Functional enrichment and correlation analyses to link DEPs with metabolic parameters.; Phenotypic characterization of Dkk3 knockout mice.
主要な成果:
- AS spermatozoa exhibit reduced oxidative phosphorylation (OXPHOS)-derived ATP, impaired electron transport chain (ETC) activity, decreased mitochondrial membrane potential (MMP), and elevated reactive oxygen species (ROS).; Proteomic analysis identified 205 DEPs, with aerobic respiration being a significantly enriched pathway.; Six DEPs, including Dickkopf (DKK)3, were significantly correlated with OXPHOS ATP and MMP.; Dkk3 knockout mice displayed reduced sperm motility and mitochondrial dysfunction, mirroring AS phenotypes.
結論:
- Mitochondrial bioenergetic impairment and elevated ROS are key features of AS.; DKK3 deficiency is implicated in AS pathogenesis, potentially through mitochondrial dysfunction.; DKK3 plays a critical role in maintaining sperm mitochondrial function and motility.
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