磁性C18修饰介孔二氧化硅作为高效吸附剂用于人血浆中强心苷的分散固相微萃取
Jia Chen1, Run-Qin Wang1, Bei-Bei Guo2
1School of Pharmacy, Shanxi Medical University, Taiyuan 030001, China; Shanxi Provincial Key Laboratory of Drug Synthesis and Novel Pharmaceutical Preparation Technology, Shanxi Medical University, Taiyuan 030001, China.
Abstract:
A magnetic mesoporous silica (SBA-15)-based dispersive solid phase microextraction was first proposed and introduced for simultaneous analysis of four cardiac glycosides (including deslanoside, lanatoside C, digoxin, and digitoxin) in human plasma coupled with HPLC-UV. In this study, magnetic C18-modified SBA-15 (SBA-15/C18/Fe3O4) was used as the extractant, which was prepared and characterized using a suite of techniques including Fourier transform infrared spectroscopy, transmission electron microscopy, N2 adsorption-desorption, vibrating sample magnetometer, scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. The influencing parameters of the extraction were investigated and optimized. The adsorption process of SBA-15/C18/Fe3O4 followed the Langmuir isotherm and pseudo-second-order kinetic models. The adsorbent also exhibited excellent thermal and chemical stability, alongside good reusability. Under the optimum experimental conditions, the proposed method demonstrated satisfactory performance with good linearity (8-3200 ng/mL for deslanoside and 1.6-640 ng/mL for lanatoside C, digoxin and digitoxin with the correlation coefficients no less than 0.9987), high precision (0.8%
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