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Subcellular Fractionation01:32

Subcellular Fractionation

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.7K
Overview of Advanced Functional Groups02:22

Overview of Advanced Functional Groups

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Functional groups are groups of atoms with specific chemical properties that occur within organic molecules and are sometimes denoted as “R”. Functional groups can “functionalize” a compound by enabling it to adopt different physical and chemical properties.
Types of Advanced Functional Groups
The table below summarizes some of the major functional groups in organic chemistry.
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Factors Influencing Attraction I: Proximity01:22

Factors Influencing Attraction I: Proximity

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Proximity plays a fundamental role in shaping interpersonal attraction by increasing opportunities for interaction and fostering familiarity. Research consistently demonstrates that individuals are more likely to form social bonds with those who are physically closer to them, whether in residential settings, workplaces, or educational institutions. This effect is largely driven by the increased frequency of encounters, which facilitates the development of friendships and romantic...
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Extraction: Advanced Methods00:56

Extraction: Advanced Methods

1.2K
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

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Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
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Updated: Feb 1, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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近接標識に基づく細胞内プロテオームマッピングの最近の進歩

Gang Wang1, Jiapeng Liu2, Xuege Sun3

  • 1College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing, 100871, China.

Molecular & cellular proteomics : MCP
|January 30, 2026
PubMed
まとめ
この要約は機械生成です。

新しい近接標識（PL）法は、細胞プロテオームを正確にマッピングし、タンパク質の局在、修飾、相互作用を明らかにします。optoPLおよびimmunoPLのような高度な技術は、詳細な細胞内プロテオーム分析のための時空間制御を提供します。

キーワード:
近接標識プロテオーム細胞内マッピングタンパク質局在ポスト翻訳修飾

さらに関連する動画

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科学分野:

  • 細胞生物学
  • プロテオミクス
  • 生化学

背景:

  • 細胞プロテオームの空間的組織化は、生理学的機能に不可欠です。
  • タンパク質の局在は、翻訳後修飾、輸送、相互作用に影響します。
  • 細胞内プロテオーム組織の理解は、タンパク質機能の知識を高めます。

研究 の 目的:

  • 近接標識（PL）技術の新興トレンドをレビューすること。
  • 高精度な細胞内プロテオームマッピングの進歩を強調すること。
  • 強化された時空間制御を提供する新しいPL法について議論すること。

主な方法:

  • 酵素媒介近接標識（PL）技術（例：TurboID、APEX2）。
  • 細胞内プロテオームマッピングのための新しいPL法のレビュー。
  • 光活性化可能なPL（optoPL）および抗体標的PL（immunoPL）の議論。

主要な成果:

  • PL技術は、生細胞における細胞内プロテオームの精密なマッピングを可能にします。
  • 新しいPL法は、多次元のタンパク質特徴（PTM、輸送、代謝、相互作用）を提供します。
  • optoPLおよびimmunoPLは、遺伝子操作なしで、強化された時空間制御と詳細なマッピングを提供します。

結論:

  • 高度なPL技術は、細胞内プロテオーム分析に革命をもたらしています。
  • これらの方法は、タンパク質の局在と機能に関する前例のない詳細を提供します。
  • 将来の研究では、これらのツールを活用して包括的なプロテオームマッピングを行うことができます。