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Updated: Feb 2, 2026

Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays

Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays

Published on: March 29, 2022

ウミイソギンチャク Bunodosoma goanense（Vennam & den Hartog, 1993）のトランスクリプトームから神経ナトリウムチャネルを標的とするペプチドNavTx-Bg1の同定と機能的特徴づけ

Cecelia Menezes¹, Narsinh Thakur², Kathleen Carleer³

¹CSIR-National Institute of Oceanography, Dona Paula, Goa, India; School of Earth, Ocean and Atmospheric Sciences, Goa University, Taleigao, Goa, India; Toxicology and Pharmacology, KU Leuven, Campus Gasthuisberg, Leuven, Belgium.

Toxicon : official journal of the International Society on Toxinology

|January 31, 2026

Abstract:

Sea anemones are a rich source of venom peptides, many of which target voltage-gated sodium channels (Nav), having immense potential for therapeutic development. In this study, we employed a transcriptomics-guided approach to predict and functionally characterize a Nav-targeting peptide from the sea anemone Bunodosoma goanense. A single putative peptide transcript with homology to known sodium channel modulators was discovered. This transcript-derived peptide was chemically synthesized and tested for activity. Electrophysiological assays employing two-electrode voltage clamp (TEVC) on Xenopus laevis oocytes overexpressing Nav1.4 and Nav1.5 channels, along with contraction paralysis assay, confirmed its functional activity. Subsequent tentacle venom purification isolated a native peptide with the same bioactivity. Our work demonstrates the power of predictive transcriptomics for sustainable venom peptide discovery.

キーワード:

ペプチドウミイソギンチャク毒電位依存性ナトリウムチャネルトランスクリプトーム解析

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