Mycobacterium bovis BCGにおける新規転写調節因子JTY_2262の調節特性
Jinmiao Song1, Hui Wang1, Mingrui Sun1
1Weifang Key Laboratory of Respiratory Tract Pathogens and Drug Therapy, School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, China.
Abstract:
Mycobacterium tuberculosis (Mtb) can survive for a long time in vivo and evade host immune attacks, primarily through transcriptional regulation mediated by transcription regulators. JTY_2262 (homologous with Rv2250c) belongs to the Tetracycline Repressor (TetR) family of regulators, and its function is currently unclear. In this study, we combined JTY_2262 overexpressed transcriptomics and electrophoretic mobility shift assays (EMSA) to identify novel targets regulated by JTY_2262. The cofactors containing Cys, VC, VB1, VB3, VB6, Pb2+, Cu2+ and Li+ inhibit the binding between JTY_2262 and the JTY_2045 (homologous with rv2031c) promoter. Overall, this study demonstrates that JTY_2262 is a versatile regulator. Under different environmental conditions, JTY_2262 senses concentration variations of specific cofactors. By modulating its DNA-binding affinity accordingly, JTY_2262 regulates targeted gene expression, enabling bacteria to adapt their metabolic machinery and physiological state to better adapt the changing environment.
関連する概念動画
Master Transcription Regulators
Master Transcription Regulators
Cooperative Binding of Transcription Regulators
Cooperative Binding of Transcription Regulators
Transcriptional Regulation: Riboswitches
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