高密度マイクロ電極アレイ上のポリジメチルシロキサン微細構造を用いたテーラード型インビトロ神経ネットワークのプロトコル

Anna-Christina Haeb¹, Hideaki Yamamoto², Paul Roach³

¹Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Institute of Neurosciences, University of Barcelona, 08036 Barcelona, Spain; Department of Condensed Matter Physics, Faculty of Physics, University of Barcelona, 08028 Barcelona, Spain; Universitat de Barcelona Institute of Complex Systems (UBICS), 08028 Barcelona, Spain; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain.

|February 1, 2026

Abstract:

Complementary metal-oxide-semiconductor (CMOS)-based high-density microelectrode arrays (HD-MEAs) enable neuronal recordings with high spatiotemporal resolution. However, integrating polydimethylsiloxane (PDMS) microstructures onto HD-MEA surfaces to control network architecture is currently challenging and platform specific. Here, we present a protocol for PDMS fabrication, HD-MEA chip preparation, PDMS-HD-MEA microstructure alignment, and cell culture, including alternatives. Our results show reproducible formation of modular networks with characteristic activity patterns across different systems. This protocol supports engineering of defined neuronal architectures while maintaining compatibility with various HD-MEA systems. For complete details on the use and execution of this protocol, please refer to Sato et al.¹.

キーワード:

生物物理学バイオテクノロジーと生物工学材料科学神経科学

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