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Updated: Feb 3, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

高パラメータスペクトルサイトメトリープロトコルによる凍結保存ヒト血液の免疫サブセットおよび機能特性評価（49バイオマーカー）

Austin P Wright¹, Marissa Lindaman², Elaine Huang²

¹Department of Cell, Development & Cancer Biology, Oregon Health & Science University and Knight Cancer Institute, Portland, OR 97239, USA; Knight Cancer Institute, Portland, OR 97201, USA.

|February 1, 2026

Abstract:

Comprehensive immune profiling of human blood is a critical approach for evaluating immune responses following therapeutic intervention in clinical trials. Here, we present a protocol to assess leukocyte lineages, activation status, and selected immune checkpoint expression in cryopreserved buffy coat using a high-dimensional, 49-color spectral flow cytometry antibody panel. We provide instructions for thawing, washing, and staining circulating immune cells, followed by guidance on data acquisition. This protocol is suitable for studying cancer immunology and other indications requiring deep immunophenotyping.

キーワード:

癌フローサイトメトリー免疫学

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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

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Published on: March 5, 2019

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

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Published on: March 6, 2018

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