機能性食品中のアントラキノンおよびフェノール酸の効果的な毛細管電気泳動分析のための新規深共晶溶媒内深共晶溶媒マイクロエマルション

This study for the first time applies a deep eutectic solvent-in-deep eutectic solvent microemulsion system as the separation medium in microemulsion electrokinetic chromatography to effectively separate an detect multiple components in Cassia seeds. The optimal experimental conditions obtained via single-factor experiments and response surface methodology are as follows: The concentration of menthol-n-octanol was 0.30%, the concentration of choline chloride-ethylene glycol is 0.60%, and the pH value is 8.49. The microemulsion contains 2% SDS and 10 mM borax. The proposed method demonstrates an excellent linear relationship, with all correlation coefficients (R²) above 0.999. The calculated detection limits and quantification limits are 0.5940-1.8987 μg/mL and 1.9802-6.3291 μg/mL, respectively. The recovery results are good, ranging from 90.68% to 107.07%. The proposed method enables the effective detection of anthraquinones and phenolic acids in food matrix, demonstrating its superior environmental friendliness and practical applicability.